E-SMDB-2046 - Transcription profiling of human mammary epithelial cells HMECs (S-FL, S-P and PGA) during replicative senescence

Status
Released on 1 November 2005, last updated on 12 October 2011
Organism
Homo sapiens
Samples (0)
Arrays (2)
Protocols (4)
Description
Telomere shortening in populations of human mammary epithelial cells (HMECs) that survive early replicative arrest (M0) by the inactivation of p16INK4A during cell culture on plastic dishes leads to a state of permanent replicative arrest termed senescence. While culture of HMECs on feeder layers abrogates M0 and p16INK4A inactivation, progressive telomere attrition in these cells also eventually results in permanent replicative arrest. Expression of telomerase prevents both senescence on plastic (S-P) and senescence on feeder layers (S-FL) in HMECs, as it does also in cultured primary human fibroblasts. We report here that the gene expression profiles of senescence in HMECs of the same lineage maintained under different culture conditions showed surprisingly little commonality. Moreover, neither of these senescence-associated profiles in HMECs resembles the profile for senescence in human fibroblasts. These results indicate that senescence-associated alterations in gene expression resulting from telomere attrition are affected by culture conditions as well as by cell origins, and argue that replicative senescence at the molecular level is a diverse rather than unique cellular process.
Experiment types
transcription profiling by array, unknown experiment type
Contacts
Citation
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-SMDB-2046.idf.txt
Sample and data relationshipE-SMDB-2046.sdrf.txt
Raw data (1)E-SMDB-2046.raw.1.zip
Processed data (1)E-SMDB-2046.processed.1.zip
Array designsA-SMDB-819.adf.txt, A-SMDB-858.adf.txt
Links