E-SMDB-1734 - Comparative genomic hybridization of pre- and post-mouse H. pylori strains SS1 and SS2000

Status
Submitted on 3 November 2005, released on 3 November 2005, last updated on 27 March 2012
Organism
Helicobacter pylori
Samples (0)
Array (1)
Description
This experiment set contains the raw data for 8 arrays that were used in the genomic typing of the pre- and post-mouse H. pylori strains SS1 and SS2000. 10700 is the pre-mouse clinical isolate of SS1 and PMSS2000 is the pre-mouse clinical isolate of SS2000. gDNA from these strains were labeled and hybridized to H. pylori microarrays as described in Salama et al. {Salama et al. 2000 PNAS 97:14668-73}. In each case the test gDNA sample was labeled with Cy5 (red) and this was hybridized with Cy3 (green) labeled reference DNA of equal amount. The reference DNA consisted of equal amounts of gDNA from the two H. pylori strains used to make the H. pylori microarray, 26695 and J99. This data was used for the manuscript: L. J. Thompson, S. J. Danon, J.E. Wilson, J. L. O'Rourke, N. R. Salama, S. Falkow, H. Mitchell, and A. Lee. (2004) Chronic Helicobacter pylori infection in C57BL/6 and BALB/c mice using SS1 and a newly identified mouse-adapted strain (SS2000). Infect. Immun. (in press).
Experiment types
comparative genomic hybridization by array, unknown experiment type
Contacts
Citation
Chronic Helicobacter pylori Infection with Sydney Strain 1 and a Newly Identified Mouse-Adapted Strain (Sydney Strain 2000) in C57BL/6 and BALB/c Mice. Thompson LJ, Danon SJ, Wilson JE, O'Rourke JL, Salama NR, Falkow S, Mitchell H, Lee A. Infect Immun 72(8):4668-79 (2004), Europe PMC 15271928
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-SMDB-1734.idf.txt
Sample and data relationshipE-SMDB-1734.sdrf.txt
Raw data (1)E-SMDB-1734.raw.1.zip
Processed data (1)E-SMDB-1734.processed.1.zip
Array designA-SMDB-46.adf.txt
Links