E-SGRP-9 - Transcription profiling of synchronized Plasmodium falciparum cultures
Submitted on 14 February 2007, released on 26 November 2007, last updated on 2 May 2014
The process of erythrocyte invasion by merozoites of Plasmodium falciparum involves multiple steps, including the formation of a moving junction characterized by the redundancy of many of the receptor-ligand interactions involved. Several of the parasite proteins that interact with erythrocyte receptors or participate in other steps of the process of invasion are encoded by small subtelomerically-located multigene families of four to seven members. We report here that members of the multigene families pfRh, eba, rhopH1/clag and acbp exist in either an active or a silenced state. In the case of two members of the rhopH1/clag family, clag3.1 and clag3.2, expression was mutually exclusive. Silencing occurred in the absence of detectable DNA alterations, suggesting that it is transmitted epigenetically. This was unambiguously demonstrated for eba-140, which was silenced by the formation of facultative heterochromatin. Our data demonstrate that variant expression, epigenetic silencing and mutually exclusive expression in Plasmodium are not unique to genes encoding proteins exported to the surface of the erythrocyte like var genes but also occur for genes involved in host cell invasion..
transcription profiling by array, co-expression, replicate, strain or line
Epigenetic silencing of Plasmodium falciparum genes linked to erythrocyte invasion. Cortes A, Carret C, Kaneko O, Yim Lim BY, Ivens A, Holder AA. PLoS Pathog 3(8):e107 (2007), Europe PMC 17676953