E-MTAB-997 - Transcription profiling by high throughput sequencing of Mbd3 knockout and wild-type mouse embryonic stem cells
Released on 4 May 2012, last updated on 6 November 2012
RNA-seq was performed for transcriptional analysis of wild-type mouse embryonic stem (ES) cells and an Mbd3 knockout line. Mbd3 is a core component of the Nucleosome Remodeling and Deacetylation (NuRD) complex, which modulates transcriptional heterogeneity and pluripotency gene expression in ES cells.
RNA-seq of coding RNA, co-expression, development or differentiation, replicate
NuRD suppresses pluripotency gene expression to promote transcriptional heterogeneity and lineage commitment. Nicola Reynolds, Paulina Latos, Antony Hynes-Allen, Remco Loos, Donna Leaford, Aoife O'Shaughnessy, Olukunbi Mosaku, Jason Signolet, Philip Brennecke, Tuzer Kalkan, Ita Costello, Peter Humphreys, William Mansfield, Kentaro Nakagawa, I. John Strouboulis, Axel Behrens, Paul Bertone, Brian Hendrich. Cell Stem Cell 10(5):538-594 (2012), Europe PMC 22560079
Zic2 is an enhancer-binding factor required for embryonic stem cell specification. Luo Z, Gao X, Lin C, Smith ER, Marshall SA, Swanson SK, Florens L, Washburn MP, Shilatifard A. :685-694 (2015), Europe PMC 25699711