E-MTAB-970 - Transcription profiling by high throughput sequencing of Sox17.Epi and Endo cells from mouse embryos

Status
Released on 20 July 2012, last updated on 3 June 2016
Organism
Mus musculus
Samples (6)
Protocols (7)
Description
This experiment has been done to understand the dynamics of gene expression in Sox17.Epi and Endo cells using multiplex RNA-Seq technology. Sox17.Epi (Sox17^GFP+EpCAM+) and Sox17.Endo (Sox17^GFP+EpCAM-) cells were isolated by FACS from E9.5 mouse embryos. The spatial comparison between Sox17.Epi versus Sox17.Endo provides insight into which genes are differentially of specifically expressed in two populations.
Experiment types
RNA-seq of coding RNA, cell type comparison design, transcription profiling by high throughput sequencing
Contacts
Citation
Dual lineage-specific expression of Sox17 during mouse embryogenesis. Choi E, Kraus MR, Lemaire LA, Yoshimoto M, Vemula S, Potter LA, Manduchi E, Stoeckert CJ Jr, Grapin-Botton A, Magnuson MA. , Europe PMC 22865702
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
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