E-MTAB-960 - microRNA profiling by array of human retinal pigment epithelial (RPE) cells to study the regulation of RPE redifferentiation
Released on 17 January 2013, last updated on 2 May 2014
Retinal Pigment Epithelial (RPE) cells are located behind the retina and are critical for photoreceptor survival. Loss of RPE is associated with several pathogenic conditions such as Age Related Macular Degeneration and Retinitis Pigmentosa. RPE derived from human embryonic stem cells (hESC) offer a potential source for producing these cells for therapy. Here we report the molecular and cellular characterization of RPE differentiated from hESC. hESC derived RPE are capable of proliferation and lose their epithelial characteristics before becoming confluent and re-differentiating back into their typical pigmented, cobblestoned appearance. During the proliferative phase, they adopt a mesenchymal morphology and express mesenchymal markers. Our results demonstrate that this apparent Epithelial-Mesenchymal Transition is not regulated by the classical EMT transcription factors SNAIL and SLUG. Furthermore, it is possible to regulate RPE de-differentiation and re-differentiation by modulating the Wnt and BMP pathway respectively. These findings further our understanding of the genesis and expansion of RPE which is essential for their therapeutic use.
microRNA profiling by array, co-expression, in vitro, time series
Characterization of hES derived RPE: RPE proliferation involves a non-classical EMT. Parul Choudhary, Christos Michaelides, Alex Gutteridge, Paul Whiting.