7 protocols
AccessionNameType
Affymetrix:Protocol:Hybridization-Unknown
hybridization protocol
Affymetrix Generic Hybridization
P-MTAB-24951
specified_biomaterial_action
At passage 7 of the HaCaT cells, cells plated in 12-well plates at 4.5e5 cells/well. A 100x stock of the chemical was freshly prepared and added to the corresponding wells, each exposure was performed in quadruplicate.
P-MTAB-24954
RMA
Samples were normalized to the average of 6 samples of the corresponding vehicle control samples, either ethanol or DMSO exposed cells. Then a log2 was applied to the data
P-MTAB-24955
image_acquisition
Scanning.
P-MTAB-24952
nucleic_acid_extraction
The RNeasy kit (Qiagen) was used according to manufacturer instructions to obtain total RNA
P-MTAB-24953
labeling
The 3'IVT Epress labeling kit was used according to manufacturer protocol (Affymetrix)
P-MTAB-24950
grow
HaCaT keratinocytes were cultured in T-flasks in DMEM medium supplemented with FCS, non-essential amino acids and penicillin/streptomicin. Around 80% confluence the cells were passaged.