E-MTAB-921 - Transcription profiling of mouse E10.5 female embryos wild-type and mutant for Smchd1
Released on 12 June 2013, last updated on 2 May 2014
To investigate the role of the Smchd1 protein in epigenetic silencing of autosomal loci, we carried out a comparative microarray gene expression analysis of wild-type and Smchd1-/- female embryos at E10.5. Embryos were collected at E10.5 after natural mating, sexed and genotyped. Total RNAs were extracted from three individual wild-type and three Smchd1-/- female embryos and cRNAs prepared from these samples was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 array.
transcription profiling by array, co-expression, in vivo, replicate
Epigenetic Functions of Smchd1 Repress Gene Clusters on the Inactive X Chromosome and on Autosomes. Anne-Valerie Gendrel; Y. Amy Tang; Masako Suzuki; Jonathan Godwin; Tatyana B. Nesterova; John M. Greally; Edith Heard; Neil Brockdorff. Mol Cell Biol 33(16):3150 (2013), Europe PMC 23754746