E-MTAB-921 - Transcription profiling of mouse E10.5 female embryos wild-type and mutant for Smchd1
Released on 12 June 2013, last updated on 31 July 2013
To investigate the role of the Smchd1 protein in epigenetic silencing of autosomal loci, we carried out a comparative microarray gene expression analysis of wild-type and Smchd1-/- female embryos at E10.5. Embryos were collected at E10.5 after natural mating, sexed and genotyped. Total RNAs were extracted from three individual wild-type and three Smchd1-/- female embryos and cRNAs prepared from these samples was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 array.
transcription profiling by array, co-expression, in vivo, replicate
Epigenetic Functions of Smchd1 Repress Gene Clusters on the Inactive X Chromosome and on Autosomes. Anne-Valerie Gendrel; Y. Amy Tang; Masako Suzuki; Jonathan Godwin; Tatyana B. Nesterova; John M. Greally; Edith Heard; Neil Brockdorff. Mol Cell Biol 33(16):3150 (2013), Europe PMC 23754746