E-MTAB-921 - Transcription profiling of mouse E10.5 female embryos wild-type and mutant for Smchd1

Status
Released on 1 April 2012, last updated on 26 October 2015
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (7)
Description
To investigate the role of the Smchd1 protein in epigenetic silencing of autosomal loci, we carried out a comparative microarray gene expression analysis of wild-type and Smchd1-/- female embryos at E10.5. Embryos were collected at E10.5 after natural mating, sexed and genotyped. Total RNAs were extracted from three individual wild-type and three Smchd1-/- female embryos and cRNAs prepared from these samples was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 array.
Experiment types
transcription profiling by array, co-expression, in vivo, replicate
Contact
Citation
Epigenetic functions of smchd1 repress gene clusters on the inactive X chromosome and on autosomes. Gendrel AV, Tang YA, Suzuki M, Godwin J, Nesterova TB, Greally JM, Heard E, Brockdorff N. , Europe PMC 23754746
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MTAB-921.idf.txt
Sample and data relationshipE-MTAB-921.sdrf.txt
Raw data (1)E-MTAB-921.raw.1.zip
Processed data (1)E-MTAB-921.processed.1.zip
Array designA-AFFY-45.adf.txt
Links