E-MTAB-921 - Transcription profiling of mouse E10.5 female embryos wild-type and mutant for Smchd1

Status
Released on 12 June 2013, last updated on 2 May 2014
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (7)
Description
To investigate the role of the Smchd1 protein in epigenetic silencing of autosomal loci, we carried out a comparative microarray gene expression analysis of wild-type and Smchd1-/- female embryos at E10.5. Embryos were collected at E10.5 after natural mating, sexed and genotyped. Total RNAs were extracted from three individual wild-type and three Smchd1-/- female embryos and cRNAs prepared from these samples was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 array.
Experiment types
transcription profiling by array, co-expression, in vivo, replicate
Contact
Citation
Epigenetic Functions of Smchd1 Repress Gene Clusters on the Inactive X Chromosome and on Autosomes. Anne-Valerie Gendrel; Y. Amy Tang; Masako Suzuki; Jonathan Godwin; Tatyana B. Nesterova; John M. Greally; Edith Heard; Neil Brockdorff. Mol Cell Biol 33(16):3150 (2013), Europe PMC 23754746
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MTAB-921.idf.txt
Sample and data relationshipE-MTAB-921.sdrf.txt
Raw data (1)E-MTAB-921.raw.1.zip
Processed data (1)E-MTAB-921.processed.1.zip
Array designA-AFFY-45.adf.txt
Links