5 protocols
AccessionNameType
P-MTAB-24568
scanning
SFF file was generated from 454 Roche machine
P-MTAB-24567
sequencing
sequencing cDNA fragments using 454 GS FLX Titanium sequencing at Wageningen UR Greenomics (Wageningen, the Netherlands). The sequence data were coded as A, T, C, G.
P-MTAB-24566
nucleic_acid_extraction
mRNA-extracted using Trizol protocol (Invitrogen. Carlsbad, CA, USA), and purified using the RNeasy MinElute kit (Qiagen, Hilden, Germany)
P-MTAB-24565
specified_biomaterial_action
Young leaves were picked and immediately frozen in liquid nitrogen and kept at -80 degree Celsius before RNA isolation
P-MTAB-24564
grow
Plants were grown in open field without any chemical treatment