5 protocols
AccessionType
scanning
SFF file was generated from 454 Roche machine
sequencing
sequencing cDNA fragments using 454 GS FLX Titanium sequencing at Wageningen UR Greenomics (Wageningen, the Netherlands). The sequence data were coded as A, T, C, G.
nucleic_acid_extraction
mRNA-extracted using Trizol protocol (Invitrogen. Carlsbad, CA, USA), and purified using the RNeasy MinElute kit (Qiagen, Hilden, Germany)
specified_biomaterial_action
Young leaves were picked and immediately frozen in liquid nitrogen and kept at -80 degree Celsius before RNA isolation
grow
Plants were grown in open field without any chemical treatment