3 protocols
AccessionType
grow
MCF10A cells were cultured with DMEM/F12 media supplemented with 2.5% Horse serum, 0.5ug/mL hydrocortisone, 10ug/mL Insulin, 20ng/mL EGF and 100ng/mL cholera toxin.
nucleic_acid_extraction
MCF10A cells were lysed in Trizol reagent (Invitrogen) and total RNA was extracted according to the manufacturer's directions.
sequencing
Total RNA was processed by the Yale Center for Genomic Analysis for Whole Transcriptome Analysis (mRNA-seq) following the instructions from Illumina, Inc.