6 protocols
AccessionType
labeling
Standard Affymetrix IVT labelling
bioassay_data_transformation
Normalization was conducted using RMAExpress software with the default settings and the data was then log2 transformed following normalization.
hybridization
Hayes A, Zhang N, Wu J, Butler PR, Hauser NC, Hoheisel JD, Lim FL, Sharrocks AD, Oliver SG: Hybridization array technology coupled with chemostat culture: Tools to interrogate gene expression in Saccharomyces cerevisiae. Methods, 2002, 26:281-290
nucleic_acid_extraction
Hayes A, Zhang N, Wu J, Butler PR, Hauser NC, Hoheisel JD, Lim FL, Sharrocks AD, Oliver SG: Hybridization array technology coupled with chemostat culture: Tools to interrogate gene expression in Saccharomyces cerevisiae. Methods, 2002, 26:281-290
specified_biomaterial_action
Homozygous deletion mutants of QDR3, PDR3 genes were grown and compared with control HO deletion mutatns
grow
Controlled and aerated chemostat cultures at 30C and pH 5.5 in defined media; glucose or ammonium limited