6 protocols
AccessionType
image_acquisition
Yale Center for Genomic Analysis performed the scanning using the Illumina scanner. Alleles were each interrogated SNP were determined using Illumina Genome Studio.
hybridization
Hybridization was performed by Yale Center for Genomic Analysis using the Human610-Quad DNA Analysis BeadChip Kit according to the manufacturer's instructions (Illumina).
labeling
The total genomic DNA was labeled using the Human610-Quad DNA Analysis BeadChip Kit according to the manufacturer's instructions (Illumina) by the Yale Center for Genomic Analysis.
grow
MCF10A cells were cultured with DMEM/F12 media supplemented with 2.5% Horse serum, 0.5ug/mL hydrocortisone, 10ug/mL Insulin, 20ng/mL EGF and 100ng/mL cholera toxin. BT474, SKBR3 and ZR75-30 were cultured in 10% FBS containing RPMI 1640 at 37C with 5% CO2
specified_biomaterial_action
nucleic_acid_extraction
Genomic DNA was extracted using Qiagen QIAamp DNA miniprep kit.