6 protocols
AccessionNameType
P-MTAB-20752
image_acquisition
Yale Center for Genomic Analysis performed the scanning using the Illumina scanner. Alleles were each interrogated SNP were determined using Illumina Genome Studio.
P-MTAB-20751
hybridization
Hybridization was performed by Yale Center for Genomic Analysis using the Human610-Quad DNA Analysis BeadChip Kit according to the manufacturer's instructions (Illumina).
P-MTAB-20750
labeling
The total genomic DNA was labeled using the Human610-Quad DNA Analysis BeadChip Kit according to the manufacturer's instructions (Illumina) by the Yale Center for Genomic Analysis.
P-MTAB-20747
grow
MCF10A cells were cultured with DMEM/F12 media supplemented with 2.5% Horse serum, 0.5ug/mL hydrocortisone, 10ug/mL Insulin, 20ng/mL EGF and 100ng/mL cholera toxin. BT474, SKBR3 and ZR75-30 were cultured in 10% FBS containing RPMI 1640 at 37C with 5% CO2
P-MTAB-20748
specified_biomaterial_action
P-MTAB-20749
nucleic_acid_extraction
Genomic DNA was extracted using Qiagen QIAamp DNA miniprep kit.