E-MTAB-651 - RNA-seq of human HCT116 cells after RNAi knock-down of signal transduction components
Released on 18 April 2014, last updated on 2 May 2014
We performed RNAi knockdown experiments on 7 different signal transduction components of the Wnt pathway in HCT116 colon cancer cells and prepared RNA libraries from the poly-adenylated fraction of the RNA. SOLiD sequencing of strand-specific, barcoded transcriptome libraries yielded expression profiles allowing us to extract common pathway signatures and enabeling us to perform susequent nested effects modelling. the resulting pathway models yields new insight in the pathway topology in colon cancer.
RNA-seq of coding RNA, cellular modification, co-expression, in vitro