E-MTAB-4787 - 5' CAGE at three embryonic times points in 82 Drosophila lines

Status
Released on 9 January 2017, last updated on 26 January 2017
Organism
Drosophila melanogaster
Samples (269)
Protocols (4)
Description
Deep sequencing of Transcriptional Start Sites (TSS) using 5' CAGE from 82 wild strains from the Drosophila Genetic reference Panel (DGRP) during embryogenesis. Three different developmental stages were assayed: 2-4hrs (stages 5-8), 6-8hrs (stages 10-11) and 10-12hrs (stages 13-14) after laying. 20 line/stage combinations were performed in biological replicates (independent embryo collections), while 5 samples were prepared as technical replicates (independent library preparation from the same RNA).
Experiment types
RNA-seq of coding RNA, development or differentiation design, population based design
Contacts
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-4787.idf.txt
Sample and data relationshipE-MTAB-4787.sdrf.txt
Links