7 protocols
AccessionType
hybridization
The biotinylated RNA was hybridized to Illumina Sentrix mouse Ref.8 V2 beadchips.
labeling
cRNA amplication and biotin labeling was performed using 500ng of total RNA with the Illumina TotalPrep TM RNA amplication kit (Ambion) according to manufacturer's protocol to produced bioin-labeled cRNAs
bioassay_data_transformation
Normexp background correction and quantile normalization using control probes (limma, Smyth 2004)
grow
The male mice were maintained on a 12-h light and dark cycle with light off at 8pm. Temperature was maintained at 20-22 C. Mice were weaned at 3 weeks and housed in groups of 10 mice in standard conditions in open top cages according to UK Home Office regulations. Mice had free access to water and standard laboratory chow pellets (B&K Universal, Hull, UK) from weaning. At 5 weeks of age, one group of mice was transferred to a 40% high fat diet (HFD) (Special Diets Services, UK), containing 32% lard oil and 8% corn oil, and a separate age matched group remained on standard laboratory chow pellets (B&K Universal, Hull, UK). Five month old male mice were killed by CO2 asphyxiation between 9 and 10 a.m. after an overnight fast.
specified_biomaterial_action
Carbohydrate diet, standard laboratory chow pellets (B&K Universal, Hull, UK).
nucleic_acid_extraction
RNEASY 96 UNIVERSAL TISSUE KIT
specified_biomaterial_action
At 5 weeks of age, one group of mice was transferred to a 40% high fat diet (HFD) (Special Diets Services, UK), containing 32% lard oil and 8% corn oil.