normexp background correction and quantile normalization using control probes (limma, Smyth 2004)
ILLUMINA TOTALPREP-96 RNA AMPLIFICATION KIT
Male mice of the 129S6 and BALB/c strains were bred locally using a stock originating from the Jackson laboratory. Hybrids of the 129S6 x BALB/c F2 cross were bred locally. All mice were maintained on a 12-h light and dark cycle with light off at 8pm. Temperature was maintained at 20-22 °C. Mice were weaned at 3 weeks and housed in groups of 10 mice in standard conditions in open top cages according to UK Home Office regulations. Mice had free access to water and standard laboratory chow pellets (B&K Universal, Hull, UK) from weaning.
RNEASY 96 Universal Tissue Kit
Carbohydrate diet, standard laboratory chow pellets (B&K Universal, Hull, UK).