E-MTAB-32 - Chromatin immunoprecipitation of adult mouse liver to identify Foxa2 targets
Released on 4 July 2008, last updated on 3 May 2014
We performed genome-wide location analysis for Foxa2 to identify its targets in the adult mouse liver. Chromatin isolated from the liver of five adult mice was cross-linked, sheared and immunoprecipitated with a Foxa2-specific antibody. The resulting material, as well as material that was not immunoprecipitated, was uncross-linked, amplified, labeled and hybridized to the Mouse Promoter Chip BCBC-5A. Statistical analysis resulted in a set of 107 genes that are bound by Foxa2. Using computational analyses, we showed that Foxa2 containing cis-regulatory modules are dependent on the strength of the Foxa2 consensus site present.
ChIP-chip by array, binding site identification, compound treatment
Cis-regulatory modules in the mammalian liver: composition depends on strength of Foxa2 consensus site. Tuteja, Geetu; Jensen, Shane T.; White, Peter; Kaestner, Klaus H. Nucleic Acids Res 36(12):4149 (2008), Europe PMC 18556755