E-MTAB-3139 - Transcriptome profiling of mouse pancreatic epithelium from Pdx1-Ngn3ER-IRES-GFP mice bred into a Neurog3-null background at embryonic day 11.25

Status
Released on 2 December 2014, last updated on 30 January 2017
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (7)
Description
Individual pancreatic buds of crosses between Ngn3+/- and Ngn3+/- ; Pdx1::Ngn3ER TM -IRES-GFP were dissected. Pregnant females were injected intra-peritoneal with 2mg of Tamoxifen at e10.25 (9 am) and dissected at e11.25 (9 to 11 am). Most of the mesenchymal tissue was removed to reduce the tissue complexity of the sample and enrich in epithelial pancreas progenitors. Moreover, at that developmental stage, Pdx1 promoter exhibits strong and homogenous expression levels. Rescuing Ngn3 expression only 24 hours before dissection (12h before activity, due to the time to process tamoxifen into the active compound 4OH tamoxifen) enriches the gene list in direct targets of Ngn3.
Experiment types
transcription profiling by array, genetic modification design
Contact
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MTAB-3139.idf.txt
Sample and data relationshipE-MTAB-3139.sdrf.txt
Raw data (1)E-MTAB-3139.raw.1.zip
Processed data (1)E-MTAB-3139.processed.1.zip
Array designA-AFFY-45.adf.txt
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