E-MTAB-2863 - Transcription profiling by array of CD11C+ splenic dendritic cells from young (8 weeks) and old (110 weeks) mice treated with Poly I:C for 4 hours
Submitted on 1 February 2013, last updated on 25 August 2014, released on 27 August 2014
Total RNA was isolated from FACS purified CD11c+ splenic DC populations isolated from young (8 weeks old, n=3) and aged (110 weeks old, n=3) mice, that had been injected with Poly I:C for 4h previously. Microarray analysis was performed with the Affymetrix GeneChip Mouse Genome 430 2.0 Arrays. The arrays were hybridized, washed and scanned according to the standard Affymetrix protocol. The gene chip tests were performed by professional staff of Shanghai Biochip Company, PRC. Gene expression profiles and functional gene set enrichment analysis demonstrate that DC isolated aged mice are impaired in their ability to activate Natural Killer (NK) cells. The study is submitted for publication in June 2014.
transcription profiling by array, organism status design
Dysfunction of dendritic cells in aged mice leads to failure of natural killer cell activation and tumor eradication. Zhenhong Guo, Tamara Tilburgs, Bonnie Wong, Jack L.Strominger.