E-MTAB-2780 - Ptf1a-dependent gene expression during pancreas development (TLDA part)

Status
Last updated on 21 July 2014, released on 15 November 2014
Organism
Mus musculus
Samples (43)
Arrays (2)
Protocols (8)
Description
We first used RNA-Seq technology to explore gene expression in mouse Ptf1a^YFP/+ [het] FACS sorted cells at E11.5 (early pancreatic Multipotent Progenitor Cells) and E15.5 (nascent acinar cells) as well as in Ptf1a^YFP/YFP [null] at E11.5 (delayed early MPC): this is deposited with accession number E-MTAB-449. We then examined 376 selected genes identified as differentially expressed between early pancreatic MPC and nascent acinar cells or between early pancreatic and delayed early MPCs by Taqman Low Density Arrays (TLDAs) with Real Time RT-PCR for each 1-day time point from E10.5 to E15. 5 in Ptf1a^YFP/+ [het] and for E10.5 and E11.5 in Ptf1aYFP/YFP [null] . Finally, 94 genes identified in the first phase of TLDAs (including 2 endogenous control, Gapdh and 18S) were analyzed in a second TLDA phase for each 1-day time point from E10.5 to -E18.5 in Ptf1a^YFP/+ [het] and for E11.5 in Ptf1aYFP/YFP [null] with biological replicates (n>=3) for each time point.
Experiment types
transcription profiling by array, development or differentiation design, genetic modification design, time series design
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MTAB-2780.idf.txt
Sample and data relationshipE-MTAB-2780.sdrf.txt
Raw data (1)E-MTAB-2780.raw.1.zip
Processed data (1)E-MTAB-2780.processed.1.zip
Additional data (1)E-MTAB-2780.additional.1.zip
Array designsA-MTAB-513.adf.txt, A-MTAB-514.adf.txt
Links