E-MTAB-2776 - Cytotoxicity Study of Rat INS1 cell lines

Status
Released on 22 July 2014, last updated on 30 July 2014
Organism
Rattus norvegicus
Samples (3)
Array (1)
Protocols (11)
Description
Cytokines IL-1 and IFN-gamma have been shown to be the primary effectors of beta-cell destruction during the immune response. The aim of this study was to identify the gene expression changes that are associated with resistance of beta cells to cytokines. INS-1 rat insulinoma cells were grown in increasing doses of cytokines until a resistance phenotype had developed. RNA was prepared from these cytokine resistant sublines as well as the original, cytokine-sensitive parental INS-1 cells in Dr. Chris Newgard's lab, quantified and sent frozen in water. 3-7 biological replicates per condition were sent for the following three conditions: (i) 834/40, Cytokine-sensitive (CS), (ii) 833/15 and 833/117, Cytokine resistant grown in the absence of cytokines (CRu), (iii) 833/15C and 833/117C: Cytokine Resistant Grown in the Presence of Cytokines (CRt). Additional files available.
Experiment types
transcription profiling by array, cell type comparison design, compound treatment design, dye swap design
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
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