E-MTAB-2736 - Equine cartilage and bone miRNA repertoires
Submitted on 31 December 2012, last updated on 23 August 2014, released on 24 August 2014
Short-read NGS technology (SOLIDTM, Life Technologies) was used to establish a comprehensive repertoire of miRNA expressed in either equine cartilage or subchondral bone. Undamaged cartilage and subchondral bone samples from 10-month Anglo-Arabian foals affected by osteochondrosis (OC) were analyzed and compared with samples from healthy foals. Samples were also subjected or not to an experimental mechanical loading to evaluate the role of miRNAs in the regulation of mechano-transduction pathways. Epiphyseal cartilage and subchondral bone miRNome were defined, including about 300 new miRNAs. Differentially expressed miRNAs were identified between bone and cartilage from healthy and OC foals, as well as after the experimental mechanical loading, suggesting that miRNAs play a role in equine OC physiopathology and in the cellular response to biomechanical stress in cartilage and bone.
RNA-seq of non coding RNA, disease state design
Next-generation sequencing identifies equine cartilage and subchondral bone miRNAs and suggests their involvement in osteochondrosis physiopathology. Desjardin C, Vaiman A, Mata X, Legendre R, Laubier J, Kennedy SP, Laloe D, Barrey E, Jacques C, Cribiu EP, Schibler L. :798 (2014), Europe PMC 25227120
Next-generation sequencing identifies equine cartilage and subchondral bone miRNAs and suggests their involvement in osteochondrosis physiopathology. Clemence DESJARDIN, Anne VAIMAN1, Xavier MATA, Rachel LEGENDRE, Johan LAUBIER, Sean P KENNEDY, Denis LALOE, Eric Barrey, Claire JACQUES, Edmond P CRIBIU and Laurent SCHIBLER.