E-MTAB-2687 - Integrin_a11_knockout
Submitted on 15 October 2012, last updated on 3 July 2014, released on 3 July 2014
The project is to study how the expression of integrin alpha11beta1 on stromal cells (MEFs) affects the tumor cell behavior (A549 cells) in a heterospheroid co-culture model. We have found that the fibroblast alpha11beta1 integrin affects compaction and interstitial fluid pressure of the heterospheroids and regulates the migration and proliferation of A549 in the heterospheroids. We then performed the gene expression profiling of the A549 cells in the alpha11+/+/A549 and alpha11-/-/A549 heterospheroids. The Microarray analysis identified CXCL5 as one molecule down-regulated in A549 cells in the absence of alpha11 on the fibroblasts. Blocking CXCL5 function reduced cell proliferation and cell migration of A549 cells within spheroids.
transcription profiling by array, genetic modification
Fibroblast α11β1 Integrin Regulates Tensional Homeostasis in Fibroblast/A549 Carcinoma Heterospheroids. Lu N, Karlsen TV, Reed RK, Kusche-Gullberg M, Gullberg D. PLoS One 9(7):e103173 (2014), Europe PMC 25076207