normalization data transformation protocol
The data was processed using dCHIP software. Significance analysis of microarrays (SAM) was used to identify differences between different samples.
nucleic acid hybridization to array protocol
The resulting RNA was used to hybridize GeneChips (Affymetrix) according to the manufacturers protocols.
nucleic acid labeling protocol
Equal aliquots of total RNA from each of four mouse livers in each group were pooled and used for biotin labeling as described in the Affymetrix technical bulletin.
Chow-fed ob/ob and C57BL/6J mice were housed in a 12 h of light and 12 h of dark cycle and fed ad libitum a regular chow diet. Mice were sacrificed at 16:00 (Zeitgeber Time 8 during light phase).
nucleic acid extraction protocol
Total RNA was prepared from each liver using TRIzol.