E-MTAB-2539 - Rpb4 functions mainly in mRNA synthesis by RNA polymerase II

Status
Released on 9 May 2014, last updated on 3 June 2014
Organism
Saccharomyces cerevisiae
Samples (7)
Array (1)
Protocols (10)
Description
RNA polymerase II (Pol II) is the central enzyme that carries out eukaryotic mRNA transcription and consists of a 10-subunit catalytic core and a heterodimeric subcomplex of subunits Rpb4 and Rpb7 (Rpb4/7). Rpb4/7 has been proposed to shuttle from the nucleus to the cytoplasm, and to function there in mRNA translation and degradation. Here we provide evidence that Rpb4 mainly functions in nuclear mRNA synthesis by Pol II, and evidence arguing against an important cytoplasmic role. We used metabolic RNA labeling and comparative Dynamic Transcriptome Analysis (cDTA) to show that Rpb4 deletion in Saccharomyces cerevisiae causes a drastic defect in mRNA synthesis that is compensated by down-regulation of mRNA degradation, resulting in mRNA level buffering. Deletion of Rpb4 can be rescued by covalent fusion of Rpb4 to the Pol II core subunit Rpb2, which largely restores mRNA synthesis and degradation defects caused by Rpb4 deletion. Thus Rpb4 is a bona fide Pol II core subunit which functions mainly in mRNA synthesis.
Experiment types
transcription profiling by array, co-expression, replicate design, strain or line design
Contact
Citation
Rpb4 functions mainly in mRNA synthesis by RNA polymerase II. Daniel Schulz, Nicole Pirkl, Elisabeth Lehmann, Patrick Cramer. Journal of Biological Chemistry  (2014)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MTAB-2539.idf.txt
Sample and data relationshipE-MTAB-2539.sdrf.txt
Raw data (1)E-MTAB-2539.raw.1.zip
Array designA-AFFY-47.adf.txt
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