E-MTAB-2536 - Utilisation of glucose by Pseudomonas putida KT2440 (PB2440) under nitrogen varying conditions

Status
Submitted on 18 December 2011, released on 24 April 2014, last updated on 3 June 2014
Organism
Pseudomonas putida
Samples (2)
Array (1)
Protocols (7)
Description
The response of P. putida KT2440 (PB2440) to preferential carbon source and varying nitrogen levels was studied in this experiment. The culture was grown in minimal medium with 2mM glucose as carbon source and different concentration of ammonium chloride as nitrogen source. For nitrogen limiting condition 2mM ammonium chloride was used whereas, for nitrogen rich condition 20mM ammonium chloride was added in the medium. Overall it was seen that the strain could grow efficiently in varying nitrogen conditions by adapting or regulating their metabolism without compromising on the growth rate. To ascertain how the cells were coping with the nitrogen stress, gene expression by microarray was performed. RNA extraction was done using Qiagen RNeasy minikit (Germany). Standard Affymetrix protocol was followed for hybridization with GeneChip P. aeruginosa Genome Array supplied by Affymetrix as GeneChip for P. putida were not supplied by any company.
Experiment types
ChIP-chip by array, growth condition design
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MTAB-2536.idf.txt
Sample and data relationshipE-MTAB-2536.sdrf.txt
Raw data (1)E-MTAB-2536.raw.1.zip
Array designA-AFFY-30.adf.txt
Links