E-MTAB-2512 - Th single cell RNA-Seq
Released on 25 April 2014, last updated on 8 September 2014
Th2 cells regulate helminth infections, allergic disorders, tumor immunity and pregnancy by secreting various cytokines. It is likely that there are undiscovered Th2-signaling molecules. While steroids are known to be immunoregulators, de novo steroid production from immune cells has not been previously characterised. We demonstrate production of the steroid pregnenolone by Th2 cells in vitro, and in vivo in a helminth infection model. To identify gene-expression identify of these steroidogenic-Th2 cells, we performed single-cell RNA-sequencing.
RNA-seq of coding RNA from single cells, cell type comparison design, co-expression, in vitro
Single Cell RNA-Sequencing Reveals T helper Cells Synthesizing Steroids de novo to Contribute to Immune Homeostasis. Bidesh Mahata, Xiuwei Zhang, Aleksandra A. Kolodziejczyk, Valentina Proserpio, Liora Haim-Vilmovsky,Angela E. Taylor, Daniel Hebenstreit, Felix A. Dingler, Victoria Moignard, Berthold Göttgens, Wiebke Arlt, Andrew N J McKenzie and Sarah A. Teichmann. Cell Rep 7(4):1130-1142 (2014), Europe PMC 24813893