E-MTAB-2508 - Transcriptional profiling of chronic myelogenous leukemia (CML) and normal, quiescent and dividing haematopoietic cells
Submitted on 15 November 2004, released on 25 April 2014, last updated on 3 June 2014
Quiescent and dividing hemopoietic stem cells (HSC) display marked differences in their ability to move between the peripheral circulation and the bone marrow. Specifically, long-term engraftment potential predominantly resides in the quiescent HSC subfraction, and G-CSF mobilization results in the preferential accumulation of quiescent HSC in the periphery. In contrast, stem cells from chronic myeloid leukemia (CML) patients display a constitutive presence in the circulation. To understand the molecular basis for this, we have used microarray technology to analyze the transcriptional differences between dividing and quiescent, normal, and CML-derived CD34+ cells.
transcription profiling by array, case control design, cell type comparison design, disease state design
Transcriptional analysis of quiescent and proliferating CD34+ human hemopoietic cells from normal and chronic myeloid leukemia sources. Graham SM, Vass JK, Holyoake TL, Graham GJ. , Europe PMC 17717066