E-MTAB-2447 - Microbiota-induced changes in Drosophila melanogaster host gene expression
Released on 1 May 2014, last updated on 3 June 2014
To understand the effects of the microbiome of Drosophila melanogaster on host gene expression, we compared the transcriptome of guts from conventionally reared flies to their axenically (germ-free)-reared counterparts. Our analysis used dissected intestines from 4-7 day-old adult females and included two wild-type fly lines, OregonR and CantonS, as well as an immune-deficient line, RelishE20. With one of the wild-type lines, CantonS, we also looked at the impact of microbiome on the transcriptional profile of dissected intestines from aged cohorts (35-40 day-old females) and young (4-7 day-old) non-gut tissues (all tissues remaining from samples dissected for the analysis of guts.
transcription profiling by array, co-expression, in vivo, stimulus or stress design
Microbiota-induced changes in Drosophila melanogaster host gene expression and gut morphology. Nichole A. Broderick, Nicolas Buchon, Bruno Lemaitre.