E-MTAB-2447 - Microbiota-induced changes in Drosophila melanogaster host gene expression

Status
Released on 1 May 2014, last updated on 3 June 2014
Organism
Drosophila melanogaster
Samples (30)
Array (1)
Protocols (7)
Description
To understand the effects of the microbiome of Drosophila melanogaster on host gene expression, we compared the transcriptome of guts from conventionally reared flies to their axenically (germ-free)-reared counterparts. Our analysis used dissected intestines from 4-7 day-old adult females and included two wild-type fly lines, OregonR and CantonS, as well as an immune-deficient line, RelishE20. With one of the wild-type lines, CantonS, we also looked at the impact of microbiome on the transcriptional profile of dissected intestines from aged cohorts (35-40 day-old females) and young (4-7 day-old) non-gut tissues (all tissues remaining from samples dissected for the analysis of guts.
Experiment types
transcription profiling by array, co-expression, in vivo, stimulus or stress design
Contact
Citation
Microbiota-induced changes in Drosophila melanogaster host gene expression and gut morphology. Nichole A. Broderick, Nicolas Buchon, Bruno Lemaitre.
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MTAB-2447.idf.txt
Sample and data relationshipE-MTAB-2447.sdrf.txt
Raw data (1)E-MTAB-2447.raw.1.zip
Array designA-AFFY-35.adf.txt
Links