7 protocols
AccessionNameType
P-AFFY-6
feature_extraction
Title: Affymetrix CEL analysis. Description:
P-AFFY-3
nucleic acid hybridization to array protocol
GeneChip hybridization
P-MTAB-38191
normalization data transformation protocol
We used Partek Genomics Suite software for probe import: Pre-background Ajustment was done for GC Content and probe sequence. We used RMA background correction and Quantile normalization. Probes were log2 transformed and probeset summarization was done with median polish.
P-MTAB-38190
nucleic acid labeling protocol
We used the Affymetrix Terminal Labeling and Controls kit for fragmentation and labeling as described by the manufacturer
P-MTAB-38189
nucleic acid extraction protocol
After cell sorting, RNA was extracted by usign the RNeasy Micro Kit (Qiagen) according to the manufacturers protocol and stored at -80C.
P-MTAB-38187
GROWTH protocol
Adult living mouse
P-MTAB-38188
treatment protocol
Isolation of lymphocytes from the lamina propria of the small intestine was performed as described previously(Sanos SL adn Diefenbach A., Methods Mol Biol. 2010;612:505-17. ). Bones were crushed with a pestle, rinsed with icecold PBS and filtered trough a cell strainer. Red cell lysis was performed. The following lymphocytes were sorted from the lamin propria of the small intestine of EomesGfg/+ RORgtCreTGg Rosa26Yfp/+ by using the markers Lineage- CD45+ Nkp46+ NK1.1+ : 1.convential NK cells (Eomes GFP+ RORgt YFP-) 2. ILC1 (Eomes GFP- RORgt YFP-) 3. exRORgt ILC3s (Eomes GFP- RORgt YFP+). Conventional NK cells from the bone marrow were sorted from Eomes Gfp/+ mice with the markers Lineage- CD45+ NK1.1+ Eomes GFP+.