6 protocols
AccessionNameType
P-MTAB-37612
array scanning and feature extraction protocol
Agilent G2565CA Microarray Scanner System with Agilent Feature Extraction software (10.7)
P-MTAB-37611
nucleic acid hybridization to array protocol
Hybridization with Agilent Gene Expression Hybridisation Kit, incubated in Agilent SureHyb chambers for 17 hours at 65°C while rotating. Washing according to manufacturer’s instructions.
P-MTAB-37610
nucleic acid labeling protocol
300 ng of total RNA was labeled with Cy3 using the Agilent Quick-Amp Labeling Kit - 1 color according to the manufacturer´s instructions
P-MTAB-37609
nucleic acid extraction protocol
RNA isolated with RNeasy Mini Kit (Qiagen) according to manufacturer's instruction
P-MTAB-37608
treatment protocol
Loading with eLDL, oxLDL and deloading with HDL3. Loading: eLDL (40 ug/mL) or oxLDL (80 ug/mL);deloading: HDL3 (100 ug/mL)
P-MTAB-37607
growth protocol
1x10^6 cells/mL in macrophage serum-free medium (Invitrogen, Germany) supplemented with 50 ng/mL recombinant human monocyte-colony stimulating factor (M-CSF; R&D Systems, USA)