E-MTAB-2175 - Molecular basis for coupling transcription termination to non-coding RNA degradation

Status
Last updated on 22 April 2014, released on 24 April 2014
Organism
Saccharomyces cerevisiae
Samples (8)
Array (1)
Protocols (5)
Description
The Nrd1-Nab3-Sen1 (NNS) complex plays a pivotal role in the control of pervasive transcription and the generation of sn- and snoRNAs in S. cerevisiae. The NNS-complex terminates transcription of non-coding RNA genes and promotes processing/degradation of transcripts by the nuclear exosome. To assess the role of the Nrd1p CTD-interacting domain (CID) in the function of the NNS-complex, we re-examined whether this domain is required for efficient transcription termination by the NNS pathway. We compared the RNA polymerase II distribution by ChIP and tiling arrays (ChIP-chip) in wild type and nrd1 deltaCID cells. Moreover, we compared the genome-wide chromatin distribution of Nrd1p in the presence and the absence of the CID by ChIP-chip analysis. ChIP of RNA polymerase II was performed using an anti-Rpb3 antibody (1Y26, Neoclone). ChIP of Nrd1 was performed using TAP-tagged S. cerevisiae strains. For details see protocols. To download the wild-type Pol II and Nrd1 data go to E-MTAB-1626 and E-MTAB-1060, respectively.
Experiment types
ChIP-chip by array, binding site identification, cellular process design, in vivo design
Contact
Citation
Molecular basis for coupling transcription termination to non-coding RNA degradation.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MTAB-2175.idf.txt
Sample and data relationshipE-MTAB-2175.sdrf.txt
Raw data (1)E-MTAB-2175.raw.1.zip
Array designA-AFFY-116.adf.txt
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