E-MTAB-2138 - Transcription profiling by array of Saccharomyces cerevisiae to gain insight into the impact of THO complex sumoylation on gene expression
Released on 25 January 2014, last updated on 3 June 2014
To gain insight into the impact of THO complex sumoylation on gene expression in yeast S. cerevisiae, total RNAs were extracted from wt and hpr1-KR cells and transcriptome profiles were analyzed by Agilent microarrays. The hpr1 K-R mutant is a version of the hpr1 THO subunit in which lysines 60-65 were simultaneously mutated, which abrogated the sumoylation of the protein.
transcription profiling by array, co-expression, dye swap design, genetic modification design
Sumoylation of the THO complex is required for optimal mRNP metabolism. Hugo BRETES, Jerome ROUVIERE, Thibaut LEGER, Marlene OEFFINGER, Frederic DEVAUX, Valerie DOYE, Benoit PALANCADE.