E-MTAB-2132 - NK CELL TOLERANCE IN LIVER TRANSPLANTATION IS RELATED TO STAT4 DOWNREGULATION

Status
Released on 1 February 2014, last updated on 3 June 2014
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (5)
Description
Liver transplantation (LT) is a definitive treatment for end-stage liver disease and hepatocellular cancer. As donor-recipient HLA matching is not employed, there is potential for natural killer (NK) cell-mediated alloreactivity. Here we report that recipient NK cells exhibit a downregulated phenotype with reduced expression of activating receptors NKp30 and NKp46. We found associated hypofunctionality, with impaired NK cell cytotoxicity, degranulation and IFN-gamma production. Gene expression analysis using microarray and quantitative PCR identified significant downregulation of STAT-4 in LT with associated reduction in miR-155, a microRNA target of STAT-4 and a key regulator of NK differentiation. These data indicate that LT induces recipient NK cell tolerance through altered peripheral maturation at a step prior to the acquisition of inhibitory receptors for HLA class I.
Experiment types
transcription profiling by array, cell type comparison design, co-expression, replicate design
Contact
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MTAB-2132.idf.txt
Sample and data relationshipE-MTAB-2132.sdrf.txt
Raw data (1)E-MTAB-2132.raw.1.zip
Array designA-MEXP-2104.adf.txt
Links