E-MTAB-2130 - Transcription profiling by array of SKM-1 cells cultured for 28 days in the absence or presence of low doses of the DNMT1 inhibitors DAC or AZA
Released on 11 December 2013, last updated on 3 June 2014
SKM-1 cells were cultured for 28 days in the absence or presence of low doses of the DNMT1 inhibitors DAC (10 nM) or AZA (100 nM). The experiment was carried in two independent set at different times, and with 2 replicates for each condition (Ctl, AZA, DAC) for a total of 12 samples. The SKM-1 cell line is derived from secondary AML arising from MDS, and is one the few representative in vitro models of MDS.
transcription profiling by array, co-expression, compound treatment design, in vitro
Prolonged treatment with DNMT inhibitors induces distinct effects in promoters and gene-bodies. Wong YF, Jakt LM, Nishikawa S.