E-MTAB-2094 - Transcriptional profiling by array of Solenopsis invicta (fire ant) workers to identify genes and gene pathways responsible for division of labor
Released on 8 January 2014, last updated on 3 June 2014
We used whole-genome fire ant microarrays to examine the molecular basis for division of labor in fire ant workers by comparing foraging and non-foraging workers from monogyne colonies. Fire ant colonies were collected in the field and transported into the lab were they were reared in standard conditions. We created a nesting chamber containing the queen, the brood and workers performing nursing tasks and a foraging area, separated from the nesting chamber and provided with food and water sources. Foraging workers were collected in the foraging area while non-foraging workers were collected in the nesting chamber. Total RNA was isolated from pools of whole workers and processed for microarrays.
transcription profiling by array, co-expression, dye swap design, innate behavior design