E-MTAB-2031 - RNA-Seq of 3iL human embryonic stem cells with an induced expression profile that more closely resembles native pluripotent cells and uninduced controls

Status
Released on 5 December 2013, last updated on 2 May 2014
Organism
Homo sapiens
Samples (2)
Protocols (5)
Description
Human embryonic stem cells (hESCs) are isolated from the inner cell mass of the blastocysts. The pluripotent properties of hESCs enable the derivation of cell-types or tissues of different lineages for potential applications such as therapeutics discovery and regenerative medicine. Even though hESCs are pluripotent, differences have been observed when compared to the native pluripotent epiblast cells of the blastocyst. We use a chemical approach (3iL: 3 small molecule inhibitor and cytokine) to induce an expression signature that more closely resembles native pluripotent cells. To better understand the difference between this hESC state and the conventional hESC state, we generated the global expression profiles using RNA-seq.
Experiment types
RNA-seq of coding RNA, cell type comparison, co-expression, in vitro
Contacts
Citation
A LIF-dependent human pluripotent state with a distinctive regulatory circuitry. Yun-Shen Chan, Jonathan Goke, Jia-hui Ng, Xinyi Lu, Cheng-Peow Tan, Wei-Quan Tng, Zhong-Zhi Hong, Huck-Hui Ng.
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-MTAB-2031.idf.txt
Sample and data relationshipE-MTAB-2031.sdrf.txt
Links