7 protocols
AccessionNameType
P-MTAB-36065
normalization data transformation protocol
We use Cufflinks to make assembly from Tophat result and Cuffidff to identify differentially expressed genes. The normalization method is RPKM
P-MTAB-36064
high throughput sequence alignment protocol
I use Tophat command line for read alignment, the reference is Aspergillus Fumigatus af293. I use command line with default parameter
P-MTAB-36063
nucleic acid sequencing protocol
TruSeq RNA sample preparation kit (Illumina)
P-MTAB-36060
treatment protocol
nothing v.s. 1 mM DTT or 10 ug/ml TM for 1 h
P-MTAB-36059
growth protocol
a 250 ml flask containing 50 ml of YG medium (0.5% yeast extract, 2% glucose) was inoculated with 5 X 107 conidia and incubated for 16 h with shaking (200 rpm) at 37 degrees C
P-MTAB-36061
nucleic acid extraction protocol
TRIZOL method
P-MTAB-36062
nucleic acid library construction protocol
Illumina HiSeq 50nt single-end reads