E-MTAB-2016 - RNA-seq of coding RNA from mycelium of fungus Podospora anserina of defined ages to study the impact of aging on gene expression

Status
Released on 20 December 2013, last updated on 2 May 2014
Organism
Podospora anserina
Samples (7)
Protocols (7)
Description
Aging of biological systems is controlled by various processes which have a potential impact on gene expression. Here we report a genome-wide transcriptome analysis of the fungal aging model Podospora anserina. Total RNA of three individuals of defined age were pooled and analyzed by SuperSAGE (serial analysis of gene expression). A bioinformatics analysis identified different molecular pathways to be affected during aging. While the abundance of transcripts linked to ribosomes and to the proteasome quality control system were found to decrease during aging, those associated with autophagy increase, suggesting that autophagy may act as a compensatory quality control pathway. Transcript profiles associated with the energy metabolism including mitochondrial functions were identified to fluctuate during aging. Comparison of wild-type transcripts, which are continuously down-regulated during aging, with those down-regulated in the long-lived, copper-uptake mutant grisea, validated the relevance of age-related changes in cellular copper metabolism. Overall, we (i) present a unique age-related data set of a longitudinal study of the experimental aging model P. anserina which represents a reference resource for future investigations in a variety of organisms, (ii) suggest autophagy to be a key quality control pathway that becomes active once other pathways fail, and (iii) present testable predictions for subsequent experimental investigations.
Experiment types
RNA-seq of coding RNA, in vitro, time series
Contacts
Citation
A Genome-Wide Longitudinal Transcriptome Analysis of the Aging Model Podospora anserina. Philipp O, Hamann A, Servos J, Werner A, Koch I, Osiewacz HD. PLoS ONE 8(12):e83109 (2013), Europe PMC 24376646
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-MTAB-2016.idf.txt
Sample and data relationshipE-MTAB-2016.sdrf.txt
Processed data (1)E-MTAB-2016.processed.1.zip
Links