E-MTAB-2005 - Methylation profiling by array of fetal human lung fibroblast cell line MRC5 over time during immortalisation with the catalytic component of telomerase (hTERT) and in vitro transformation with SV40 Large T antigen and oncogenic H-RAS

Status
Released on 11 November 2013, last updated on 3 June 2014
Organism
Homo sapiens
Samples (32)
Array (1)
Protocols (10)
Description
Analysis of DNA methylation changes at promoters in the fetal human lung fibroblast cell line MRC5 over time (two time points: 50 and 100 population doublings) during immortalisation with the catalytic component of telomerase (hTERT) and in vitro transformation with SV40 Large T antigen and oncogenic H-RAS
Experiment types
methylation profiling by array, development or differentiation design, genetic modification design, genotyping design, reference design
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Citation
Immortality, but not oncogenic transformation, of primary human cells leads to epigenetic reprogramming of DNA methylation and gene expression. Gordon, Katrina; Clouaire, Thomas; Bao, Xun; Kemp, Sadie; Xenophontos, Maria; de las Heras, Jose; Stancheva, Irina.
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