E-MTAB-1998 - RNA-seq of lung and hippocampus tissues from reciprocal cross C57BL/6J x B6(C)-H2bm12/KhEgJ mice to find genes whose expression is altered in trans depending on whether the mother or father carried a knockout allele for H2-ab1

Status
Released on 1 November 2013, last updated on 2 May 2014
Organism
Mus musculus
Samples (60)
Protocols (6)
Description
We looked for parent of origin effects on genome-wide gene expression in a reciprocal cross between C57BL/6J x B6(C)-H2bm12/KhEgJ mice (the latter strain is a knockout of H2-ab1 on a C57BL/6J background). The aim is to find genes whose expression is altered in trans depending on whether the mother or father carried a knockout allele for H2-ab1. In this experiment differential expression of a gene is inferred from a difference in its overall expression levels. We sequenced RNA from 30 F1 mice from the H2-ab1 heterozygous cross. Both heterozygous and wild type F1 animals were sequenced in order to find both parental and parent of origin effects. We measured expression in the lungs (average 6.2 million reads per sample) and hippocampus (6.6 million reads).
Experiment types
RNA-seq of coding RNA, ex vivo, individual genetic characteristics
Contact
Citation
The Architecture of Parent-of-Origin Effects in Mice. Richard Mott, Wei Yuan, Pamela Kaisaki, Xiangchao Gan, James Cleak, Andrew Edwards, Amelie Baud, Jonathan Flint. cell 156(1):332-342 (2014)
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-MTAB-1998.idf.txt
Sample and data relationshipE-MTAB-1998.sdrf.txt
Processed data (1)E-MTAB-1998.processed.1.zip
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