E-MTAB-1945 - Transcription profiling by array of budding yeast with or without Msn2 to look for Msn2-regulated genes
Released on 12 October 2013, last updated on 3 June 2014
Transcriptional profiling of Msn2 regulated genes. Both strains are PKAas (TPK1M164G TPK2M147G TPK3M165G) and msn4-delta. One strain has Msn2 C-terminally tagged with mCherry and the other strain is msn2-delta. In this experiment, both strains were exposed to 3 uM 1-NM-PP1, which leads to Msn2 nuclear localization, for 0, 10, 20 and 40 min. The goal was to determine the genes that show a strong upregulation in the presence of Msn2, but no expression change in the absence of Msn2. Results from MNase-sequencing study of nucleosome remodelling over a Msn2 expression time-course were also deposited at ArrayExpress under accession number E-MTAB-1950 ( https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-1950/ ).
transcription profiling by array, co-expression, ex vivo, time series
Promoter decoding of transcription factor dynamics involves a trade-off between noise and control of gene expression. Anders S. Hansen, Erin K. O'Shea. Mol Syst Biol 9:704 (2013), Europe PMC 24189399