E-MTAB-1906 - Transcription profiling by array of primary human adipose stromal cells from rs4684847 CC risk allele carriers cultured and induced to differentiate into adipocytes, and simultaneously transfected for with non-targeting siRNA or siRNA targeting PRRX1 or both PRRX1 and PPARG

Status
Submitted on 4 September 2013, released on 1 December 2013, last updated on 3 June 2014
Organism
Homo sapiens
Samples (10)
Array (1)
Protocols (6)
Description
Primary human adipose stromal cells (hASCs) from rs4684847 CC risk allele carriers were cultured and induced to differentiate into adipocytes, and simultaneously transfected for 72h with non-targeting siRNA or siRNA targeting PRRX1 (n=10) or both PRRX1 and PPARG (subset of the subjects, n=4).
Experiment types
transcription profiling by array, cellular modification design
Contacts
Kjell Petersen <kjell.petersen@bccs.uib.no>, Bernhard Horsthemke, Bernward Klocke, Christoph Hoffmann, Derek Spieler, DIAGRAM+ Consortium, Gunnar Mellgren, Hans Hauner, Harald Grallert, Heekyoung Lee, Helmut Laumen, Ingrid Dahlman, Joao Fadista, Karsten Suhre, Kerstin Ehlers, Kun Qian, Leif Groop, Lena Riess, Martin Klingenspor, Martin Seifert, Martina Muller-Nurasyid, Matthias Bluher, Melina Claussnitzer, Michael J. Kern, Nancy Bretschneider, Niklas Mejhert, Nikolay Oskolkov, Ola Hansson, Peter Arner, Simon N. Dankel, Simone Wahl, Stefanie M. Hauck, Tea Berulava, Thomas Illig, Thomas Skurk, Tina Ronn, Viktoria Glunk, Yi-Hsiang Hsu
Citation
Leveraging Cross-Species Transcription Factor Binding Site Patterns: From Diabetes Risk Loci to Disease Mechanisms. Melina Claussnitzersend, Simon N. Dankel, Bernward Klocke, Harald Grallert, Viktoria Glunk, Tea Berulava, Heekyoung Lee, Nikolay Oskolkov, Joao Fadista, Kerstin Ehlers, Simone Wahl, Christoph Hoffmann, Kun Qian, Tina Rönn, Helene Riess, Martina Müller-Nurasyid, Nancy Bretschneider, Timm Schroeder, Thomas Skurk, Bernhard Horsthemke, DIAGRAM+Consortium, Derek Spieler, Martin Klingenspor, Martin Seifert, Michael J. Kern, Niklas Mejhert, Ingrid Dahlman, Ola Hansson, Stefanie M. Hauck, Matthias Blüher, Peter Arner, Leif Groop, Thomas Illig, Karsten Suhre, Yi-Hsiang Hsu, Gunnar Mellgren, Hans Hauner, Helmut Laumensend. Cell 156(1-2):343-358 (2014)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MTAB-1906.idf.txt
Sample and data relationshipE-MTAB-1906.sdrf.txt
Raw data (1)E-MTAB-1906.raw.1.zip
Processed data (1)E-MTAB-1906.processed.1.zip
Array designA-MEXP-2072.adf.txt
Links