6 protocols
AccessionNameType
P-MTAB-35538
high throughput sequence alignment protocol
LifeScope Software v2.5 was used for alignment of sequence reads
P-MTAB-35539
normalization data transformation protocol
Custom scripts were used to quantify the fraction of intronic reads in each of the RNA-seq datasets
P-MTAB-35537
nucleic acid sequencing protocol
The samples were barcoded and sequenced on the SOLiD5500W System, 75bp read lengths
P-MTAB-35534
growth protocol
Tissue and total RNA samples from two fetal frontal cortex tissues, 38 weeks female (Sample 1) and 23/24 weeks male (Sample 2) were purchased from Capital Biosciences. The commercial adult frontal lobe polyA+ RNA sample with two rounds of polyA+ selection was acquired from Clontech (Catalogue number: 636165).
P-MTAB-35535
nucleic acid extraction protocol
Cytoplasmic RNA, nuclear RNA, total RNA and PolyA+ RNA was extracted from each of the samples. A novel protocol for cytoplasmic and nuclear RNA extraction was developed, based on the Norgen purification kit.
P-MTAB-35536
nucleic acid library construction protocol
The SOLiD RNA-seq protocol was used to prepare RNA libraries