E-MTAB-1898 - RNA-seq of pure fractions of nuclear and cytoplasmic nascent or mature RNA with or without polyA enrichment from different cellular compartments

Status
Released on 22 October 2013, last updated on 3 June 2014
Organism
Homo sapiens
Samples (9)
Protocols (6)
Description
The aim of this experiment was to develop new methods to extract pure fractions of nuclear and cytoplasmic RNA. We compared the results of nuclear and cytoplasmic RNA-sequencing to results of the total and polyA+ RNA-sequencing. Cytoplasmic, nuclear, total and polyA+ RNA was extracted from two human brain samples and sequenced on the SOLiD system. We analyzed the data to look for differences in levels of nascent transcription and mature mRNAs between the different samples.
Experiment types
RNA-seq of non coding RNA, cell type comparison
Contact
Citation
Efficient cellular fractionation improves RNA sequencing analysis of mature and nascent transcripts from human tissues. Ammar Zaghlool, Adam Ameur, Linnea Nyberg, Manfred Grabherr, Lucia Cavelier, Lars Feuk.
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
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