E-MTAB-1887 - Transcription profiling by array of zebrafish with conditional heatshock induced overexpression of transgenic wnt8b or transgenic dkk1b to study the wnt signalling pathway in zebrafish development

Status
Last updated on 14 November 2013, released on 14 November 2013
Organism
Danio rerio
Samples (8)
Array (1)
Protocols (7)
Description
To investigate its role during zebrafish development, wnt signalling was either activated by the conditional expression of wnt8b or inhibited by the conditional expression of dickkopf-1b (dkk1b) under the control of the hsp70 heat shock promoter. The transgenic constructs contained a gfp reporter fusion to differentiate between transgenic embryos and their wild type siblings. Transgenic embryos and wild type control embryos at the 48 hours post fertilization stage (hpf) were exposed to heat shock (37 C) for 2 hours and were collected 4 hours after heat shock. The transcriptional response to activated or inhibited wnt signalling was profiled using microarrays and compared to wild type expression.
Experiment types
transcription profiling by array, co-expression, replicate, stimulus or stress
Contact
Citation
A bi-modal function of Wnt signalling directs an FGF activity gradient to spatially regulate neuronal differentiation in the midbrain. Carlene Dyer, Eric Blanc, Anja Hanisch, Henry Roehl, Georg W. Otto, Tian Yu, M. Albert Basson and Robert Knight. Development 141:1-10 (2014)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MTAB-1887.idf.txt
Sample and data relationshipE-MTAB-1887.sdrf.txt
Raw data (1)E-MTAB-1887.raw.1.zip
Processed data (1)E-MTAB-1887.processed.1.zip
Array designA-MEXP-1926.adf.txt
Links