5 protocols
AccessionNameType
P-MTAB-34864
nucleic acid sequencing protocol
Illumina GAII, v4 chemistry, v2 cluster generation kit. Sequences were called using IBIS base caller (Kircher et al. 2009).
P-MTAB-34861
treatment protocol
Transfection of plasmid containing the RUNX2 gene with Amaxa Nucleofector electroporation
P-MTAB-34863
nucleic acid library construction protocol
All cDNA samples were prepared as described in the Illumina Inc. manual Small RNA Sample Preparation Guide (Part # 1004239 Rev. A Illumina Inc. San Diego).
P-MTAB-34862
nucleic acid extraction protocol
Nucleic acid extraction using RNeasy kit
P-MTAB-34860
growth protocol
All cell lines were cultured as recommended by the supplier.