nucleic acid sequencing protocol
Illumina GAII, v4 chemistry, v2 cluster generation kit. Sequences were called using IBIS base caller (Kircher et al. 2009).
Transfection of plasmid containing the RUNX2 gene with Amaxa Nucleofector electroporation
nucleic acid library construction protocol
All cDNA samples were prepared as described in the Illumina Inc. manual Small RNA Sample Preparation Guide (Part # 1004239 Rev. A Illumina Inc. San Diego).
nucleic acid extraction protocol
Nucleic acid extraction using RNeasy kit
All cell lines were cultured as recommended by the supplier.