7 protocols
normalization data transformation protocol
Rank-invariant normalization method
array scanning and feature extraction protocol
Signal is developed with streptavidin-Cy3 and the BeadChip is scanned with the Illumina BeadArray Reader. WGGE_DH_Assay Guide 11286331
nucleic acid hybridization to array protocol
The labeled RNA is then hybridized to the array at 58C overnight, and then washed the following day. WGGE_DH_Assay Guide 11286331
nucleic acid labeling protocol
Illumina’s recommended sample labeling procedure starts with unlabeled total RNA extracted from a eukaryotic sample, and produces an amplified pool of biotin-labeled RNA corresponding to the polyadenylated (mRNA) fraction. WGGE_DH_Assay Guide 11286331
nucleic acid extraction protocol
The rats were decapitated under anesthesia with ethyl ether 3 and 24 h after the operation. The frontoparietal cortex of the ischemic animals was isolated.
treatment protocol
The irreversible electrical coagulation of the distal segment of the left middle cerebral artery was performed under anesthesia with chloral hydrate (300 ug/kg)
treatment protocol
During the experiment, ischemia + Semax animals were given intraperitoneal injections of Semax (100 ug/kg), whereas ischemia animals were injected with saline (100 ul/100g of 0.9% NaCl). The injections of Semax or saline were performed 15 min, 1, 4 and 8 h after occlusion