7 protocols
AccessionNameType
P-MTAB-34568
normalization data transformation protocol
Rank-invariant normalization method
P-MTAB-34567
array scanning and feature extraction protocol
Signal is developed with streptavidin-Cy3 and the BeadChip is scanned with the Illumina BeadArray Reader. WGGE_DH_Assay Guide 11286331
P-MTAB-34566
nucleic acid hybridization to array protocol
The labeled RNA is then hybridized to the array at 58C overnight, and then washed the following day. WGGE_DH_Assay Guide 11286331
P-MTAB-34565
nucleic acid labeling protocol
Illumina’s recommended sample labeling procedure starts with unlabeled total RNA extracted from a eukaryotic sample, and produces an amplified pool of biotin-labeled RNA corresponding to the polyadenylated (mRNA) fraction. WGGE_DH_Assay Guide 11286331
P-MTAB-34564
nucleic acid extraction protocol
The rats were decapitated under anesthesia with ethyl ether 3 and 24 h after the operation. The frontoparietal cortex of the ischemic animals was isolated.
P-MTAB-34562
treatment protocol
The irreversible electrical coagulation of the distal segment of the left middle cerebral artery was performed under anesthesia with chloral hydrate (300 ug/kg)
P-MTAB-34563
treatment protocol
During the experiment, ischemia + Semax animals were given intraperitoneal injections of Semax (100 ug/kg), whereas ischemia animals were injected with saline (100 ul/100g of 0.9% NaCl). The injections of Semax or saline were performed 15 min, 1, 4 and 8 h after occlusion