E-MTAB-1856 - Ribonucleoprotein immunoprecipitation microarray (RIp-chip) study with Exo2 and Eti1 proteins

Status
Released on 5 May 2014, last updated on 3 June 2014
Organism
Schizosaccharomyces pombe
Samples (2)
Array (1)
Protocols (13)
Description
We have used RIp-chip to identify mRNAs that coprecipitae with specific proteins, and later used the protein RNA interactions to predict protein-protein associations. To test whether some of the interactions we see require intact polysomes (that is, ribosomes translating mRNAs) we treated the extracts (and, in some cases, also the cells) with different compounds. In this case, we have a standard protocol, one in which the buffers contain EDTA, and one in which it includes puromycin.
Experiment types
ChIP-chip by array, RIP, binding site identification design
Contact
Citation
Cotranslational protein-RNA associations predict protein-protein interactions. Caia Duncan and Juan Mata.
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-MTAB-1856.idf.txt
Sample and data relationshipE-MTAB-1856.sdrf.txt
Raw data (1)E-MTAB-1856.raw.1.zip
Processed data (1)E-MTAB-1856.processed.1.zip
Array designA-MEXP-2049.adf.txt
Links