E-MTAB-1856 - Ribonucleoprotein immunoprecipitation microarray (RIp-chip) study with Exo2 and Eti1 proteins
Released on 5 May 2014, last updated on 3 June 2014
We have used RIp-chip to identify mRNAs that coprecipitae with specific proteins, and later used the protein RNA interactions to predict protein-protein associations. To test whether some of the interactions we see require intact polysomes (that is, ribosomes translating mRNAs) we treated the extracts (and, in some cases, also the cells) with different compounds. In this case, we have a standard protocol, one in which the buffers contain EDTA, and one in which it includes puromycin.
ChIP-chip by array, RIP, binding site identification design
Cotranslational protein-RNA associations predict protein-protein interactions. Caia Duncan and Juan Mata.